Coding

Part:BBa_K4623010:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)


Twisted Silinker(GFP),The process of protein coiling can be visualized and green fluorescence can be


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1099
    Illegal SapI.rc site found at 1528


Design Notes

The sequence includes the stop codon


Source

This part sequence is primarily derived from an artificially modified amino acid sequence that has been optimized based on E. coli codons.

References